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asn168Download scientific diagram | Mutation of Ala1, Asn168 and Cys+1 in MIP. Left panel: mutation of Cys+1 blocks splicing and formation of the DTT-cleavable branched thioester intermediate,Intact N-glycans at Asn168 seem to protect DAO from rapid clearance in rodents. Full processing of all glycoforms is critical for preserving the improved in vivo half-life